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Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates
JoVE Journal
Cancer Research
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JoVE Journal Cancer Research
Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

Analytical Determination of Mitochondrial Function of Excised Solid Tumor Homogenates

DOI:
10.3791/62875-v

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11:32 min

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August 06, 2021

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, , ,
1Integrated Physiology and Molecular Medicine Laboratory,Pennington Biomedical Research Center, 2Clinical Oncology and Metabolism,Pennington Biomedical Research Center, 3Department of Nutrition,Case Western Reserve University, 4Department of Clinical Nutrition,University of Texas Southwestern Medical Center

Chapters

  • 00:04Introduction
  • 00:54Tumor Homogenate Preparation
  • 04:27Substrate, Uncoupler, Inhibitor Titration Protocol (SUIT)
  • 09:00Results: Mitochondrial Function of Excised Solid Tumors Homogenates
  • 10:52Conclusion

Summary

Automatic Translation

Automatic Translation

We developed a practical protocol and analytical approach to evaluate mitochondrial oxidative phosphorylation and electron transfer capacity in fresh tumor homogenates. This protocol can be easily adapted to survey various mitochondrial functions that contribute to cancer initiation, progression, and treatment response.

Tags

Keywords: Mitochondrial FunctionTumor HomogenatesMitochondrial RespirationBiopsy PreservationQuantitative AssessmentClinical Translational OncologyDiagnostic InterpretationCancer Progression

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