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Fluorescensaktiveret cellesortering-radioligandbehandlet væv (FACS-RTT) til bestemmelse af den cellulære oprindelse af radioaktivt signal
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1Division of Adult Psychiatry, Department of Psychiatry,University Hospitals of Geneva, 2Department of Psychiatry,University of Geneva, 3Division of Nuclear medicine and Molecular Imaging,University Hospitals of Geneva, 4Division of Radiation Oncology, Department of Oncology,University Hospitals of Geneva, 5Department of Brain Sciences, Faculty of Medicine,Imperial College London
Chapters
- 00:04Introduction
- 00:38SPECT Camera Preparation and Calibration
- 01:47[125I]CLINDE Radiotracer Synthesis
- 04:27SPECT Acquisition
- 05:15Scan Reconstruction
- 05:57Rat Brain Extraction and Cell Isolation
- 09:46Results: FACS-RTT to Determine the Cellular Origin of Radioactive Signal
- 11:27Conclusion
Fluorescensaktiveret cellesortering-radioligandbehandlet væv (FACS-RTT) er et kraftfuldt værktøj til at studere rollen som 18 kDa translocatorprotein eller Serotonin 5HT 2A-receptorekspression i Alzheimers sygdom i cellulær skala. Denne protokol beskriver ex vivo-anvendelsen af FACS-RTT i TgF344-AD-rottemodellen.
