JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Developmental Biology
/
原位
斑马鱼幼体和幼鱼在雌雄同体发育过程中的杂交
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Developmental Biology
In Situ
Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development
Please note that all translations are automatically generated.
Click here for the English version.
原位
斑马鱼幼体和幼鱼在雌雄同体发育过程中的杂交
DOI:
10.3791/62930-v
•
08:16 min
•
August 13, 2021
•
Sagar N. Kasar
,
Sarah A. Grandinette
,
Scott D. Semelsberger
,
Cuong Q. Diep
1
Department of Biology
,
Indiana University of Pennsylvania
Chapters
00:04
Introduction
00:32
Raising Larvae
01:03
Days 1-2: Fixing Larvae and Day 3: Measuring Larvae
02:16
Days 3 4: Dehydration and Day 5: Rehydration, Proteinase K Digestion, Bleaching and Prehybridization
04:05
Day 6: Probe Hybridization and Day 7: Probe Washing and Blocking
05:38
Days 8 9: Antibody Incubation and Days 10 11: Antibody Washing
06:05
Day 12: Staining and Imaging
07:18
Results: In Situ Hybridization for Gene Expression Analysis in Zebrafish Larvae and Juveniles During Mesonephros Development
07:52
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
斑马鱼是了解肾脏发育的重要模型。在这里,
对原位
杂交方案进行了优化,以检测斑马鱼幼虫和幼鱼在中胚层发育过程中的基因表达。
Tags
In Situ Hybridization
Zebrafish Larvae
Zebrafish Juveniles
Mesonephros Development
Gene Expression
Probe Penetration
Kidney Visualization
Mating
Embryo Collection
Larva Fixation
Larva Dehydration
Larva Rehydration
Proteinase K Digestion
Bleaching
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
了解早期器官使用简体<em>原位</em>杂交协议<em>爪</em
捕获组织修复的斑马鱼幼虫与时间推移明视立体显微镜
斑马鱼幼虫骨骼肌诚信与伊文思蓝分析
诱导生活青蛙和斑马鱼胚胎缺氧
全贴装荧光
原位
杂交和免疫荧光法在斑马鱼中 Multiciliated 细胞的可视化
阿斯蒂亚纳克斯
胚胎的全原位杂交
斑马鱼原位杂交染色的基因分型与定量
斑马鱼胚胎全山原位杂交和管状测定在iPSC-ECs中研究恩多林在血管发育中的作用
斑马鱼胚胎和幼虫的整个山免疫性化学
荧光钙成像及其后续原位杂交,用于
异种性
前体特征
Read Article