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얼룩말 물고기 애벌레의 척추 모터 뉴런에서 TDP-43의 광유전학 적 단계 전환
JoVE Journal
Neuroscience
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JoVE Journal Neuroscience
Optogenetic Phase Transition of TDP-43 in Spinal Motor Neurons of Zebrafish Larvae
DOI:

07:14 min

February 25, 2022

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Chapters

  • 00:04Introduction
  • 01:36Preparation of Light Emitting Diode (LED) for Blue Light Illumination
  • 02:13Imaging of Zebrafish Larvae Expressing Optogenetic TAR DNA-binding Protein 43 (TDP-43)
  • 03:58Light Stimulation of Optogenetic TDP-43h-Expressing Fish by Field Illumination of a Blue LED Light
  • 04:35Results: Characterization of TDP-43 Phase Transition in Spinal Motor Neurons of Zebrafish Larvae
  • 06:34Conclusion

Summary

Automatic Translation

우리는 제브라피시를 모델로 사용하여 척추 운동 뉴런의 빛에 의한 TAR DNA 결합 단백질(43)의 위상 전이를 유도하는 프로토콜을 설명합니다.

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