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Biology
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DNA双链断裂时转录活性的活细胞成像
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Live-Cell Imaging of Transcriptional Activity at DNA Double-Strand Breaks
Please note that all translations are automatically generated.
Click here for the English version.
DNA双链断裂时转录活性的活细胞成像
DOI:
10.3791/62968-v
•
09:07 min
•
September 20, 2021
•
Madalena R. de Almeida
,
Eduardo Gameiro
,
Sérgio F. de Almeida
,
Robert M. Martin
1
Instituto de Medicina Molecular João Lobo Antunes
,
Faculdade de Medicina da Universidade de Lisboa
Chapters
00:04
Introduction
00:53
Preparation and Transfection of Cells for Live-Cell Microscopy
02:09
Experimental Setup
04:05
Image Acquisition
06:04
Microscopy Calibration Measurements and Analysis
06:51
Results: Analysis of Transcription Detection at Sites of DNA Double-Strand Breaks
08:12
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
该协议提出了一种新的报告基因系统和实验设置,以检测具有单分子灵敏度的DNA双链断裂处的转录。
Tags
Live-cell Imaging
Transcriptional Activity
DNA Double-strand Breaks
Transcription Detection
Single-cell Analysis
DNA Damage Response
Transcription-replication Crosstalk
Doxycycline Induction
Calibration Measurements
Microcentrifuge Tube Preparation
Transfection
Glass Bottom Dish
Microscopy Observation
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