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Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung
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Immunology and Infection
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JoVE Journal Immunology and Infection
Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

Flow Cytometric Analysis for Identification of the Innate and Adaptive Immune Cells of Murine Lung

DOI:
10.3791/62985-v

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09:57 min

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November 16, 2021

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, , , ,
1Division of Hematology-Oncology,Beth Israel Deaconess Medical Center, Harvard Medical School, 2Department of Medicine,Beth Israel Deaconess Medical Center, Harvard Medical School, 3Harvard College

Chapters

  • 00:04Introduction
  • 01:17Surgical Excision and Tissue Preparation
  • 02:11Preparation of Single-Cell Suspension
  • 04:12Surface Antibody Staining
  • 05:25Cell Fixation and Intracellular Staining
  • 06:46Results: Gating Strategy
  • 09:10Conclusion

Summary

Automatic Translation

Automatic Translation

In this study, we present an effective and reproducible protocol to isolate the immune populations of the murine respiratory system. We also provide a method for the identification of all innate and adaptive immune cells that reside in the lungs of healthy mice, using a 9-color-based flow cytometry panel.

Tags

Lung Immune CellsMyeloid CellsNon-myeloid CellsFlow CytometryGating StrategyLung Immune Landscape

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