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Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model
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Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

Evaluation of Hepatic Glucose Production in a Polycystic Ovary Syndrome Mouse Model

DOI:
10.3791/62991-v

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09:44 min

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March 05, 2022

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, , , ,
1Department of Reproductive Endocrinology and Infertility,Baylor College of Medicine, 2Family Fertility Center,Texas Children’s Hospital, 3Children’s Nutrition Research Center,Baylor College of Medicine, 4Critical Care Medicine, Department of Pediatrics,Baylor College of Medicine

Chapters

  • 00:04Introduction
  • 01:00Preparation of [6,6-2H2]glucose
  • 02:15Set-up of Infusion Experiments
  • 03:19Catheter Insertion
  • 04:24Infusion Set-up and First Infusion
  • 05:51Blood Sampling
  • 06:30Second Infusion
  • 07:13Analyses and Calculations
  • 08:07Results
  • 08:39Conclusion

Summary

Automatic Translation

Automatic Translation

This study describes the direct measurement of hepatic glucose production in a polycystic ovary syndrome mouse model by using a stable isotopic glucose tracer via tail vein in both fasting and glucose-rich states in tandem.

Tags

Hepatic Glucose ProductionPolycystic Ovary SyndromePCOSStable Isotopic Glucose TracerGas Chromatography-mass SpectrometryGCMSFastingGlucose-rich ConditionInfusionC57BL6J Mice

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