Automating Aggregate Quantification in Caenorhabditis elegans

Alfonso S. Vaziriyan-Sani; Robert D. Handy; Alyssa C. Walker; Carol Navya Pagolu; Samantha M. Enslow; Daniel   M. Czy&#380;

doi:10.3791/62997

Journal

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Biology

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Automatisere aggregert kvantifisering i Caenorhabditis elegans

JoVE Journal
Biology

This content is Free Access.

JoVE Journal Biology

Automating Aggregate Quantification in Caenorhabditis elegans

Automatisere aggregert kvantifisering i Caenorhabditis elegans

DOI:

10.3791/62997-v

•

07:50 min

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October 14, 2021

•

Alfonso S. Vaziriyan-Sani, Robert D. Handy, Alyssa C. Walker, Carol Navya Pagolu², Samantha M. Enslow, Daniel M. Czyż

¹Department of Microbiology and Cell Science,University of Florida, ²Department of Computer and Information Science and Engineering,University of Florida

Chapters

00:04Introduction
02:28Image Analysis
05:38Results: The Analysis of Aggregate Detection and the Aggregate Quantification Efficacy Using CellProfiler and Automation

Summary

Automatic Translation

Følgende protokoll beskriver utvikling og optimalisering av en arbeidsflyt med høy gjennomstrømning for ormdyrking, fluorescensavbildning og automatisert bildebehandling for å kvantifisere polyglutaminaggregater som en vurdering av endringer i proteostase.