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Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

Cell-Free Scaled Production and Adjuvant Addition to a Recombinant Major Outer Membrane Protein from Chlamydia muridarum for Vaccine Development

DOI:
10.3791/63028-v

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12:53 min

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March 16, 2022

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, , , , , , , , , , ,
1Physical and Life Sciences Directorate,Lawrence Livermore National Laboratory, 2Department of Pathology and Laboratory Medicine,University of California, 3School of Medicine, Radiation Oncology,University of California Davis

Chapters

  • 00:04Introduction
  • 01:00Cell‐Free Production of MOMP‐tNLPs for Subunit Vaccine Formulations
  • 04:21MOMP‐tNLP Purification
  • 07:19Western and Dot Blots and Storage
  • 10:14Results: SDS‐PAGE Analysis
  • 11:46Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes using commercial, cell-free protein expression kits to produce membrane proteins supported in nanodisc that can be used as antigens in subunit vaccines.

Tags

Keywords: Cell-free ExpressionRecombinant ProteinMembrane ProteinMOMPChlamydiaVaccine DevelopmentAdjuvantLipid NanoparticleScalable Production

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