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Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence
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Cancer Research
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JoVE Journal Cancer Research
Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

Monitoring Breast Cancer Growth and Metastatic Colony Formation in Mice using Bioluminescence

DOI:
10.3791/63060-v

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06:52 min

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November 05, 2021

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, , ,
1Department of Biochemistry and Molecular Biology, The Institute for Medical Research Israel-Canada,The Hebrew University-Hadassah Medical School

Chapters

  • 00:04Introduction
  • 00:50Validating In Vitro Luciferase Activity
  • 01:55Injecting Mice with GFP+ Luc+ Cells
  • 02:50Measuring the Luciferase Levels in GFP+ Luc+ Mice
  • 04:43Results: Detection of Tumor Development and Metastatic Colonization In Real-Time
  • 06:05Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we describe a noninvasive monitoring method involving luciferase and green fluorescent protein expression in various breast cancer cell lines. This protocol provides a technique to monitor tumor formation and metastatic colonization in real time in mice.

Tags

BioluminescenceBreast CancerMetastasisTumor GrowthCell LinesLuciferaseIn Vivo ImagingMouse ModelPreclinical Studies

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