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Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator
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Neuroscience
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JoVE Journal Neuroscience
Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

Live Imaging of the Mitochondrial Glutathione Redox State in Primary Neurons using a Ratiometric Indicator

DOI:
10.3791/63073-v

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07:47 min

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October 20, 2021

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, , ,
1Department of Medical Cell Biology, Institute for Anatomy and Cell Biology,Heidelberg University, 2Department of Neurogenetics,Max-Planck-Institute for Experimental Medicine

Chapters

  • 00:05Introduction
  • 00:55Optimization of Scanning Confocal Microscope Settings
  • 01:58Live Imaging of the Mitochondrial Redox Response to N-methyl-D-aspartate (NMDA) Treatment
  • 03:08Data Analysis
  • 05:40Results: Effects of N-methyl-D-aspartate (NMDA) on Neuronal Mitochondria Oxidation
  • 07:04Conclusion

Summary

Automatic Translation

Automatic Translation

This article describes a protocol to determine differences in basal redox state and redox responses to acute perturbations in primary hippocampal and cortical neurons using confocal live microscopy. The protocol can be applied to other cell types and microscopes with minimal modifications.

Tags

Keywords: Mitochondrial Glutathione Redox StateLive ImagingPrimary NeuronsRatiometric IndicatorMitochondrial Redox StateMitochondrial Membrane PotentialCalcium ConcentrationsConfocal MicroscopyNMDADTT

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