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非酒精性脂肪性肝炎小鼠模型中肝细胞外基质的 3D 成像

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Medicine

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JoVE Journal Medicine

3D Imaging of the Liver Extracellular Matrix in a Mouse Model of Non-Alcoholic Steatohepatitis

Please note that all translations are automatically generated. Click here for the English version.

非酒精性脂肪性肝炎小鼠模型中肝细胞外基质的 3D 成像

DOI:

10.3791/63106-v

•

06:46 min

•

February 25, 2022

•

Weiguo Fan, Yuan Li, Koshi Kunimoto, Natalie J. Török

¹Gastroenterology and Hepatology,Stanford University

Chapters

00:04Introduction
00:46Surgical Preparation and Procedures of Liver Perfusion
02:08Tissue Decellularization and Slide Preparation for Imaging
03:35Image Acquisition
04:58Results: Two-Photon Microscopy of the Liver Extracellular Matrix in a Mouse
06:19Conclusion

Summary

Automatic Translation

本协议优化了肝脏原位灌注/脱细胞和双光子显微镜方法，以建立一个可靠的平台来可视化非酒精性脂肪性肝炎（NASH）期间细胞外基质（ECM）重塑的动力学。

Tags

Keywords: 3D Imaging Liver Extracellular Matrix Mouse Model Non-alcoholic Steatohepatitis NASH Fibrosis Cirrhosis Liver Cancer ECM Remodeling Portal Vein Catheterization Perfusion Sodium Deoxycholate

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