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Kvantificering af vaskulære parametre i hele Mount Retinas af mus med ikke-proliferative og proliferative retinopatier
JoVE Journal
Neuroscience
Author Produced
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JoVE Journal Neuroscience
Quantification of Vascular Parameters in Whole Mount Retinas of Mice with Non-Proliferative and Proliferative Retinopathies
DOI:

12:28 min

March 12, 2022

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Chapters

  • 00:13Introduction
  • 01:19Fluorescent Lectin Statining
  • 03:31Confocal Microscopy Visualization and Microphotograph Acquisition
  • 04:14Image Processing
  • 05:31Quantification of Avascular Areas
  • 06:01Quantification of Neovascular Areas
  • 06:27Quantification of Vessel Diameter
  • 06:57Quantification of Vessel Tortuosity
  • 07:44Quantification of Vascular Branching
  • 08:16Quantification of Vascular Density
  • 08:43Results
  • 12:00Conclusion

Summary

Automatic Translation

Denne artikel beskriver en veletableret og reproducerbar lectin plet assay for hele mount retinal præparater og de protokoller, der kræves for den kvantitative måling af vaskulære parametre ofte ændret i proliferative og ikke-proliferative retinopatier.

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