Journal
/
Immunology and Infection
/
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2
JoVE Journal
Immunology and Infection
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Immunology and Infection
Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

Live Imaging and Quantification of Viral Infection in K18 hACE2 Transgenic Mice Using Reporter-Expressing Recombinant SARS-CoV-2

DOI:
10.3791/63127-v

•

08:41 min

•

November 05, 2021

•

, , , , ,
1Texas Biomedical Research Institute

Chapters

  • 00:05Introduction
  • 01:15Bioluminescence Monitoring K18 hACE2 Transgenic Mice Infected with rSARS-CoV-2/Nluc
  • 02:24Fluorescence Analysis in K18 hACE2 Transgenic Mice Infected with rSARS-CoV-2/Venus
  • 03:49Viral Titrations
  • 05:48Results: rSARS-CoV-2Nluc and rSARS-CoV-2/Venus Expression in Infected K18 Hace2 Transgenic Mice
  • 08:12Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol describes the dynamics of viral infections using luciferase- and fluorescence-expressing recombinant (r)SARS-CoV-2 and an in vivo imaging systems (IVIS) in K18 hACE2 transgenic mice to overcome the need of secondary approaches required to study SARS-CoV-2 infections in vivo.

Tags

Live ImagingQuantificationViral InfectionK18 HACE2 Transgenic MiceReporter-expressing Recombinant SARS-CoV-2In VivoEx VivoFluorescenceBioluminescenceLuciferaseIVISLungsNecropsy

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63127?article_type=v

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host
check_url/63127?article_type=v

Production and Titering of Recombinant Adeno-associated Viral Vectors
check_url/63127?article_type=v

A Luciferase-fluorescent Reporter Influenza Virus for Live Imaging and Quantification of Viral Infection
check_url/63127?article_type=v

Visualization of SARS-CoV-2 using Immuno RNA-Fluorescence In Situ Hybridization
check_url/63127?article_type=v

Detection of SARS-CoV-2 Neutralizing Antibodies using High-Throughput Fluorescent Imaging of Pseudovirus Infection
check_url/63127?article_type=v

A Rapid, Multiplex Dual Reporter IgG and IgM SARS-CoV-2 Neutralization Assay for a Multiplexed Bead-Based Flow Analysis System
check_url/63127?article_type=v

Detection of SARS-CoV-2 Receptor-Binding Domain Antibody using a HiBiT-Based Bioreporter
check_url/63127?article_type=v

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
check_url/63127?article_type=v

Studying Host-Virus Interactions with Pseudotyped Viruses (Video) | JoVE
check_url/63127?article_type=v

Author Spotlight: A Pseudotype Virus System for Assessing Omicron Subvariants and Neutralizing Antibodies in SARS-CoV-2 Research

Read Article