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シングルセルマルチオミクス実験のためのマウス頸動脈内腔からの内皮細胞の単離
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Biology
Isolation of Endothelial Cells from the Lumen of Mouse Carotid Arteries for Single-Cell Multi-Omics Experiments
Please note that all translations are automatically generated.
Click here for the English version.
シングルセルマルチオミクス実験のためのマウス頸動脈内腔からの内皮細胞の単離
DOI:
10.3791/63128-v
•
09:12 min
•
October 04, 2021
•
Sandeep Kumar*
1
,
Aitor Andueza*
1
,
Nicolas Villa-Roel
,
Juyoung Kim
,
Dong-Won Kang
,
Hanjoong Jo
2
1
Wallace H. Coulter Department of Biomedical Engineering
,
Georgia Institute of Technology and Emory University
,
2
Division of Cardiology
,
Georgia Institute of Technology and Emory University
Chapters
00:05
Introduction
00:51
Partial Carotid Ligation (PCL) Surgery
01:57
Isolation of Carotid Arteries Post Sacrifice
03:02
Endothelial-Cell-Enriched Single-Cell Isolation from Mice Carotids
04:02
Flushing the Carotid Arteries
05:53
Single-Cell and Single-Nucleus Analyses
07:18
Results: Standard QC Metrics for the scRNAseq and scATACseq Study
08:34
Conclusion
Summary
Automatic Translation
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Automatic Translation
我々は、1細胞オミクス実験を行うために、安定したまたは乱れた流動条件にさらされたマウス頸動脈の内腔から内皮細胞および核を単離する方法を提示する。
Tags
Keywords: Endothelial Cells
Carotid Artery
Single-cell Multi-omics
Atherosclerosis
Blood Vessel
Perfusion
Digestion Buffer
Ligation
Dissection
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