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Isoler celletypespecifikke RNA'er fra snapfrosne heterogene vævsprøver uden cellesortering

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Biology

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JoVE Journal Biology

Isolate Cell-Type-Specific RNAs from Snap-Frozen Heterogeneous Tissue Samples without Cell Sorting

Isoler celletypespecifikke RNA'er fra snapfrosne heterogene vævsprøver uden cellesortering

DOI:

10.3791/63143-v

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08:30 min

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December 08, 2021

•

Huifei Sophia Zheng, Chen-Che Jeff Huang

¹Department of Anatomy, Physiology and Pharmacology, College of Veterinary Medicine,Auburn University

Chapters

00:05Introduction
00:43Tissue Lysis and Homogenization
01:31Immunoprecipitation
02:30RNA Extraction
05:31Results: Microarray and qPCR Analysis of TRAP Samples
07:45Conclusion

Summary

Automatic Translation

Denne protokol sigter mod at isolere celletypespecifik oversættelse af ribosomale mRNA'er ved hjælp af NuTRAP-musemodellen.

Tags

Keywords: Cell-type-specific RNA Ribosome-bound RNA EGFP GFP Antibody Protein G Beads RNA Isolation RNA Purification

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