Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
JoVE Journal
Developmental Biology

A subscription to JoVE is required to view this content. Sign in or start your free trial.

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis
 

Ex Utero Culture of Mouse Embryos from Pregastrulation to Advanced Organogenesis

Article DOI: 10.3791/63160-v 07:14 min October 19th, 2021
October 19th, 2021

Chapters

Summary

An enhanced platform for whole-embryo culture allows continuous and robust ex utero development of postimplantation mouse embryos for up to six days, from pregastrulation stages until advanced organogenesis. In this protocol, we detail the standard procedure for successful embryo culture using static plates and rotating bottle systems.

Tags

Keywords: Ex Utero Culture Mouse Embryos Pregastrulation Organogenesis Gastrulation Roller Culture Incubator Gas Concentration Gas Pressure Embryo Dissection Culture Medium DPBS Pregnant Mouse
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter