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Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity
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Developmental Biology
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JoVE Journal Developmental Biology
Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

Stab Wound Injury Model of the Adult Optic Tectum Using Zebrafish and Medaka for the Comparative Analysis of Regenerative Capacity

DOI:
10.3791/63166-v

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06:12 min

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February 10, 2022

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,
1Biomedical Research Institute,National Institute of Advanced Industrial Science and Technology, 2DBT-AIST International Laboratory for Advanced Biomedicine,National Institute of Advanced Industrial Science and Technology

Chapters

  • 00:04Introduction
  • 00:55Stab Wound Injury in the Adult Optic Tectum
  • 01:34Brain Dissection
  • 03:01Preparation of Frozen Sections
  • 04:44Results: Fluorescent Immunostaining Against RGC Proliferation and the Generation of Newborn Neurons After Tectum Injury
  • 05:28Conclusion

Summary

Automatic Translation

Automatic Translation

A mechanical brain injury model in the adult zebrafish is described to investigate the molecular mechanisms regulating their high regenerative capacity. The method explains to create a stab wound injury in the optic tectum of multiple species of small fish to evaluate the regenerative responses using fluorescent immunostaining.

Tags

Keywords: Stab Wound InjuryOptic TectumZebrafishMedakaRegenerative CapacityBrain InjuryRGC ProliferationDifferentiationNeedle-mediated InjuryCentral Nervous SystemRegenerative TherapyPBS PerfusionParaformaldehyde Fixation

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