Waiting
Login processing...

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Genetics / Determining the Role of Maternally-Expressed…
JoVE Journal
Genetics

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Genetics
Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants
 

Determining the Role of Maternally-Expressed Genes in Early Development with Maternal Crispants

Article DOI: 10.3791/63177-v • 10:08 min • December 21st, 2021
December 21st, 2021 •
1, 1, 1
1Laboratory of Genetics, University of Wisconsin-Madison

Chapters

Summary
Automatic Translation

Early development is dependent on maternally-inherited products, and the role of many of these products is currently unknown. Herein, we described a protocol that uses CRISPR-Cas9 to identify maternal-effect phenotypes in a single generation.

Tags

Keywords: Maternal CRISPR Maternal Effect Genes Early Development Embryogenesis Guide RNA In-vitro Transcription Zebrafish Embryo Injection
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter