Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Takunori Minegishi; Ryosuke Fujikawa; Ria Fajarwati Kastian; Yuichi Sakumura; Naoyuki Inagaki

doi:10.3791/63227

Journal

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Neuroscience

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Analyser af Actin Dynamics, Kobling Kobling og Traction Force for Vækst Cone Advance

JoVE Journal
Neuroscience

This content is Free Access.

JoVE Journal Neuroscience

Analyses of Actin Dynamics, Clutch Coupling and Traction Force for Growth Cone Advance

Analyser af Actin Dynamics, Kobling Kobling og Traction Force for Vækst Cone Advance

DOI:

10.3791/63227-v

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07:53 min

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October 21, 2021

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Takunori Minegishi, Ryosuke Fujikawa, Ria Fajarwati Kastian, Yuichi Sakumura, Naoyuki Inagaki

¹Laboratory of Systems Neurobiology and Medicine, Division of Biological Science,Nara Institute of Science and Technology, ²Laboratory of Data-Driven Biology, Division of Biological Science,Nara Institute of Science and Technology

Chapters

00:04Introduction
00:35Single Speckle Imaging at Neuronal Growth Cones
02:06Traction Force Microscopy at Neuronal Growth Cones
03:46Quantification of Traction Force
06:01Results: Fluorescence Images and Force Mapping of a Neuronal Growth Cone
07:19Conclusion

Summary

Automatic Translation

For at fremme skal vækst kegler udøve trækkraft mod det ydre miljø. Generationen af trækkraft er afhængig af actin dynamik og kobling kobling. Den foreliggende undersøgelse beskriver metoder til analyse actin dynamik, kobling kobling og trækkraft kræfter for vækst kegle forhånd.

Analyser af Actin Dynamics, Kobling Kobling og Traction Force for Vækst Cone Advance

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Chapters

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