Journal
/
Biology
/
Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies
JoVE Journal
Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

Tobacco Hornworm as an Insect Model System for Cannabinoid Pre-clinical Studies

DOI:
10.3791/63228-v

•

05:25 min

•

December 29, 2021

•

, , , , ,
1Institute of Cannabis Research,Colorado State University-Pueblo, 2Department of Biology,Colorado State University-Pueblo, 3Department of Chemistry,Colorado State University-Pueblo, 4Chuncheon Bioindustry Foundation

Chapters

  • 00:05Introduction
  • 00:39Hornworm Preparation and Cannabidiol Treatment
  • 02:03M. sexta Larval Growth, Diet Consumption, and Mortality Measurements
  • 03:07Results: Effects of Cannabidiol (CBD) on the Growth, Mortality Feeding Behavior, and Mortality of Tobacco Hornworm Manduca sexta
  • 04:18Conclusion

Summary

Automatic Translation

Automatic Translation

The present protocol provides instructional information for using tobacco hornworm Manduca sexta in cannabinoid research. The method described here includes all necessary supplies and protocols to monitor physiological and behavioral changes of the insect model in response to cannabidiol (CBD) treatment.

Tags

Keywords: Tobacco HornwormInsect Model SystemCannabinoidPreclinical StudiesCannabidiolLarval GrowthDiet ConsumptionLarval Mobility

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63228?article_type=v

Genetic Studies of Human DNA Repair Proteins Using Yeast as a Model System
check_url/63228?article_type=v

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism
check_url/63228?article_type=v

Efficient Production and Purification of Recombinant Murine Kindlin-3 from Insect Cells for Biophysical Studies
check_url/63228?article_type=v

An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
check_url/63228?article_type=v

Imaging Spatial Reorganization of a MAPK Signaling Pathway Using the Tobacco Transient Expression System
check_url/63228?article_type=v

Establishment of an Extracellular Acidic pH Culture System
check_url/63228?article_type=v

Experimental Protocol for Using Drosophila As an Invertebrate Model System for Toxicity Testing in the Laboratory
check_url/63228?article_type=v

Mass Spectrometry Analysis to Identify Ubiquitylation of EYFP-tagged CENP-A (EYFP-CENP-A)
check_url/63228?article_type=v

Preparing and Injecting Embryos of Culex Mosquitoes to Generate Null Mutations using CRISPR/Cas9
check_url/63228?article_type=v

Cryo-Electron Microscopic Grid Preparation for Time-Resolved Studies using a Novel Robotic System, Spotiton

Read Article