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Modellering Paracrine Noncanonical Wnt Signalering In Vitro
JoVE Journal
Developmental Biology
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JoVE Journal Developmental Biology
Modeling Paracrine Noncanonical Wnt Signaling In Vitro
DOI:

11:14 min

December 10, 2021

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Chapters

  • 00:04Introduction
  • 00:36Cell Plating in Co‐Culture System
  • 02:50Wound Assay and Quantitative Assessment of Myoblast Migration
  • 05:57Immunofluorescence Staining and Imaging of Migrating Myoblasts
  • 08:29Results: Effects of Wnt5a and ROR2 Knockdown on Myoblast Migration and Morphology
  • 10:28Conclusion

Summary

Automatic Translation

Den aktuella studien beskriver en mycket reproducerbar och lätthanterlig metod för att studera parakrina icke-kanoniska Wnt-signaleringshändelser in vitro. Detta protokoll tillämpades för att utvärdera effekten av parakrin Wnt5a-signalering i murina neurala vapenceller och myoblaster.

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