Journal
/
Developmental Biology
/
Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae
JoVE Journal
Developmental Biology
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Developmental Biology
Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

Using Alizarin Red Staining to Detect Chemically Induced Bone Loss in Zebrafish Larvae

DOI:
10.3791/63251-v

•

05:03 min

•

December 28, 2021

•

, , , , , , , ,
1Department of Toxicology, School of Public Health, Jiangsu Key Laboratory of Preventive and Translational Medicine for Geriatric Diseases,Medical College of Soochow University, 2Department of Occupational Disease Prevention,Jiangsu Provincial Center for Disease Control and Prevention, 3Jiangsu Preventive Medicine Association, Nanjing, China

Chapters

  • 00:04Introduction
  • 00:36Alizarin Red Staining
  • 02:05Image Acquisition
  • 02:38Image Analysis
  • 03:49Results: Bone Loss Detection Using Alizarin Red Staining
  • 04:36Conclusion

Summary

Automatic Translation

Automatic Translation

Here, we have used alizarin red staining to show that lead acetate exposure causes a bone mass change in zebrafish larvae. This staining method can be adapted to the investigation of bone loss in zebrafish larvae loss induced by other hazardous toxicants.

Tags

Keywords: Alizarin Red StainingZebrafish LarvaeBone ToxicityOsteoporosisSkeletal ChangesChemical ExposureImage AnalysisBone Mass Quantification

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

check_url/63251?article_type=v

Capturing Tissue Repair in Zebrafish Larvae with Time-lapse Brightfield Stereomicroscopy
check_url/63251?article_type=v

Analysis of Zebrafish Larvae Skeletal Muscle Integrity with Evans Blue Dye
check_url/63251?article_type=v

Microstructured Devices for Optimized Microinjection and Imaging of Zebrafish Larvae
check_url/63251?article_type=v

Adult Zebrafish Injury Models to Study the Effects of Prednisolone in Regenerating Bone Tissue
check_url/63251?article_type=v

Genotyping and Quantification of In Situ Hybridization Staining in Zebrafish
check_url/63251?article_type=v

Whole Mount Immunohistochemistry in Zebrafish Embryos and Larvae
check_url/63251?article_type=v

Using Flow Cytometry to Detect and Quantitate Altered Blood Formation in the Developing Zebrafish
check_url/63251?article_type=v

Using Immunofluorescence to Detect PM2.5-induced DNA Damage in Zebrafish Embryo Hearts
check_url/63251?article_type=v

Light-Induced GFP Expression in Zebrafish Embryos using the Optogenetic TAEL/C120 System
check_url/63251?article_type=v

In Situ Hybridization in Zebrafish Larvae and Juveniles during Mesonephros Development

Read Article