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Journal
/
Biology
/
对成年
冈比亚按蚊
的有效口服RNA干扰(RNAi)给药
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Effective Oral RNA Interference (RNAi) Administration to Adult
Anopheles gambiae
Mosquitoes
Please note that all translations are automatically generated.
Click here for the English version.
对成年
冈比亚按蚊
的有效口服RNA干扰(RNAi)给药
DOI:
10.3791/63266-v
•
07:48 min
•
March 01, 2022
•
Mabel Taracena
2
,
Catherine Hunt
,
Pamela Pennington
,
Deborah Andrew
5
,
Marcelo Jacobs-Lorena
6
,
Ellen Dotson
,
Michael Wells
7,8
1
Division of Parasitic Diseases and Malaria, Entomology Branch
,
Centers for Disease Control and Prevention
,
2
Department of Entomology
,
Cornell University
,
3
Centro de Estudios en Biotecnologia
,
Universidad del Valle de Guatemala
,
4
Department of Cell Biology
,
Johns Hopkins School of Medicine
,
5
Johns Hopkins Malaria Research Institute
,
Johns Hopkins Bloomberg School of Public Health
,
6
Department of Molecular Microbiology and Immunology
,
Johns Hopkins Bloomberg School of Public Health and Malaria Research Institute
,
7
Department of Cell Biology
,
Johns Hopkins School of Medicine
,
8
Biomedical Sciences Department
,
Idaho College of Osteopathic Medicine
Chapters
00:05
Introduction
00:45
Preparation of Heat-Killed Bacteria Expressing Double-Stranded RNA (dsRNA)
02:09
Feeding Mosquitoes with Heat-Killed Bacteria Expressing dsRNA
02:47
Assay Target Gene Expression Levels
03:59
Phenotypic Evaluation: Successful Blood-Feeding
04:35
Phenotypic Evaluation: Salivary Gland Morphology and Down-Regulation of Relevant Proteins
05:30
Results: The Effects of
fkh
Knockdown in Adult
Anopheles gambiae
Salivary Glands
07:17
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
口服由细菌产生的dsRNA,一种常规用于
秀丽隐杆线虫
的RNA干扰(RNAi)递送方法,在这里成功地应用于成年蚊子。我们的方法允许在不使用注射的情况下进行稳健的反向遗传学研究和传播阻断载体研究。
Tags
RNA Interference
RNAi
Oral Delivery
Anopheles Gambiae
Mosquitoes
Double-stranded RNA
Gene Silencing
Reverse Genetics
Bacterial Culture
IPTG Induction
Heat-killed Bacteria
Sugar Solution
Cotton Ball Feeder
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