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Chapters
- 00:04Introduction
- 01:04Multiphoton Fluorescence Lifetime Imaging (FLIM) of Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) and Flavin Adenine Dinucleotide (FAD)
- 02:13Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) Imaging
- 03:35Flavin Adenine Dinucleotide (FAD) Imaging
- 04:45Cyanide Experiment Preparation
- 05:28Results: The Fluorescence Imaging of MCF-7 Cells Before and After Cyanide Treatment
- 07:05Conclusion
Denne protokol beskriver fluorescensbilleddannelse og analyse af de endogene metaboliske coenzymer, reduceret nicotinamid adenin (phosphat) dinukleotid (NAD (P) H) og oxideret flavin adenin dinukleotid (FAD). Autofluorescensbilleddannelse af NAD (P) H og FAD giver en etiketfri, ikke-destruktiv metode til vurdering af cellulær metabolisme.
