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Chapters
- 00:04Introduction
- 01:04Multiphoton Fluorescence Lifetime Imaging (FLIM) of Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) and Flavin Adenine Dinucleotide (FAD)
- 02:13Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) Imaging
- 03:35Flavin Adenine Dinucleotide (FAD) Imaging
- 04:45Cyanide Experiment Preparation
- 05:28Results: The Fluorescence Imaging of MCF-7 Cells Before and After Cyanide Treatment
- 07:05Conclusion
Denne protokollen beskriver fluorescensavbildning og analyse av endogene metabolske koenzymer, redusert nikotinamid adenin (fosfat) dinukleotid (NAD(P)H) og oksidert flavin adenin dinukleotid (FAD). Autofluorescence imaging av NAD (P)H og FAD gir en etikettfri, ikke-ødeleggende metode for å vurdere cellulær metabolisme.
