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Chapters
- 00:04Introduction
- 01:04Multiphoton Fluorescence Lifetime Imaging (FLIM) of Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) and Flavin Adenine Dinucleotide (FAD)
- 02:13Nicotinamide Adenine (Phosphate) Dinucleotide (NAD(P)H) Imaging
- 03:35Flavin Adenine Dinucleotide (FAD) Imaging
- 04:45Cyanide Experiment Preparation
- 05:28Results: The Fluorescence Imaging of MCF-7 Cells Before and After Cyanide Treatment
- 07:05Conclusion
Detta protokoll beskriver fluorescensavbildning och analys av endogena metaboliska koenzymer, reducerad nikotinamidadenin (fosfat) dinukleotid (NAD (P) H) och oxiderad flavinadenindinukleotid (FAD). Autofluorescensavbildning av NAD(P)H och FAD ger en etikettfri, icke-destruktiv metod för att bedöma cellulär metabolism.
