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Bioengineering
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Karakterisering af blodudvækstendotelceller (BOEC) fra perifert blod fra svin
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood
Karakterisering af blodudvækstendotelceller (BOEC) fra perifert blod fra svin
DOI:
10.3791/63285-v
•
08:02 min
•
January 06, 2022
•
Akankshya Shradhanjali
,
Susheil Uthamaraj
,
Dan Dragomir-Daescu
,
Rajiv Gulati
,
Gurpreet S. Sandhu
,
Brandon J. Tefft
1
Department of Biomedical Engineering
,
Medical College of Wisconsin & Marquette University
,
2
Division of Engineering
,
Mayo Clinic
,
3
Department of Physiology & Biomedical Engineering
,
Mayo Clinic
,
4
Department of Cardiovascular Medicine
,
Mayo Clinic
Chapters
00:04
Introduction
00:37
Collection of Porcine Peripheral Blood
02:36
Isolation of Mononuclear Cells
03:33
Washing and Plating of Cells
05:16
Cell Culture
05:50
Results: Morphological Differentiation, Organization and Characterization of Blood Outgrowth Endothelial Cells (BOECs)
07:27
Conclusion
Tags
Keywords: Blood Outgrowth Endothelial Cells
BOEC
Porcine
Peripheral Blood
Cell Therapy
Disease Modeling
Heparin
Femoral Vein
Femoral Artery
Density Gradient
Centrifugation
Cell Culture
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