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Journal
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Bioengineering
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Caractérisation des cellules endothéliales de croissance sanguine (BOEC) à partir du sang périphérique porcin
JoVE Journal
Bioengineering
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JoVE Journal
Bioengineering
Characterization of Blood Outgrowth Endothelial Cells (BOEC) from Porcine Peripheral Blood
Please note that all translations are automatically generated.
Click here for the English version.
Caractérisation des cellules endothéliales de croissance sanguine (BOEC) à partir du sang périphérique porcin
DOI:
10.3791/63285-v
•
08:02 min
•
January 06, 2022
•
Akankshya Shradhanjali
,
Susheil Uthamaraj
,
Dan Dragomir-Daescu
,
Rajiv Gulati
,
Gurpreet S. Sandhu
,
Brandon J. Tefft
1
Department of Biomedical Engineering
,
Medical College of Wisconsin & Marquette University
,
2
Division of Engineering
,
Mayo Clinic
,
3
Department of Physiology & Biomedical Engineering
,
Mayo Clinic
,
4
Department of Cardiovascular Medicine
,
Mayo Clinic
Chapters
00:04
Introduction
00:37
Collection of Porcine Peripheral Blood
02:36
Isolation of Mononuclear Cells
03:33
Washing and Plating of Cells
05:16
Cell Culture
05:50
Results: Morphological Differentiation, Organization and Characterization of Blood Outgrowth Endothelial Cells (BOECs)
07:27
Conclusion
Tags
Blood Outgrowth Endothelial Cells
BOEC
Porcine
Peripheral Blood
Cell Therapy
Disease Modeling
Heparin
Femoral Vein
Femoral Artery
Density Gradient
Centrifugation
Cell Culture
Article
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