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转录组水平上人类原发性肠系膜动脉内皮细胞的分离和分析

JoVE Journal
Medicine

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JoVE Journal Medicine

Isolation and Profiling of Human Primary Mesenteric Arterial Endothelial Cells at the Transcriptome Level

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转录组水平上人类原发性肠系膜动脉内皮细胞的分离和分析

DOI:

10.3791/63307-v

•

09:45 min

•

March 14, 2022

•

Naseeb Kaur Malhi*¹, Yingjun Luo*¹, Xiaofang Tang*¹, Kiran Sriram³, Riccardo Calandrelli, Sheng Zhong, Zhen Bouman Chen³

¹Department of Diabetes Complications and Metabolism,City of Hope, ²Department of Bioengineering,University of California San Diego, ³Irell and Manella Graduate School of Biological Sciences,City of Hope

Chapters

00:04Introduction
01:28Physical Dissociation
03:26Preparation for RNA-Seq Studies
04:28Spatial Transcriptome Profiling
07:13Results: Analysis of Endothelial Cells (ECs)
08:52Conclusion

Summary

Automatic Translation

该方案描述了从人肠系膜动脉中分离、培养和分析内皮细胞。此外，还提供了一种制备用于空间转录组学的人类动脉的方法。蛋白质组学，转录组学和功能测定可以在分离的细胞上进行。该方案可以重新用于任何中型或大型动脉。

Tags

Endothelial Cells Mesenteric Artery Transcriptome Single-cell Spatial Resolution Diabetes Obesity Cryostat Sectioning Cell Isolation Digestion Buffer Cell Culture

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