/

/

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

JoVE Journal
Neuroscience

A subscription to JoVE is required to view this content. Sign in or start your free trial.

JoVE Journal Neuroscience

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

Modeling Neonatal Intraventricular Hemorrhage Through Intraventricular Injection of Hemoglobin

DOI:

10.3791/63345-v

•

07:57 min

•

August 25, 2022

•

Brandon A. Miller², Shelei Pan, Peter H. Yang, Catherine Wang, Amanda L. Trout, Dakota DeFreitas, Sruthi Ramagiri, Scott D. Olson, Jennifer M. Strahle^4,5

¹Department of Neurosurgery,University of Kentucky, ²Department of Pediatric Surgery,University of Texas, ³Department of Neurological Surgery,Washington University in St. Louis School of Medicine, ⁴Department of Orthopedic Surgery,Washington University in St. Louis School of Medicine, ⁵Department of Pediatrics,Washington University in St. Louis School of Medicine

Chapters

00:04Introduction
01:03Preparation of the Animal for Injection
02:01Setting up the Stereotactic Injector
03:05Animal Injection
04:33MRI Acquisition and Imaging
05:44Results: Induction of Intraventricular Hemorrhage by Hemoglobin Injection
07:20Conclusion

Summary

Automatic Translation

We present a model of neonatal intraventricular hemorrhage using rat pups that mimics the pathology seen in humans.

Tags

Keywords: Neonatal Intraventricular Hemorrhage IVH Hemoglobin Injection Hydrocephalus Brain Injury Stereotactic Injection Animal Model Pathophysiology Post-hemorrhagic Hydrocephalus Neurological Sequelae

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

Autologous Blood Injection to Model Spontaneous Intracerebral Hemorrhage in Mice

Direct Intraventricular Delivery of Drugs to the Rodent Central Nervous System

Neonatal Pial Surface Electroporation

Intracerebroventricular Viral Injection of the Neonatal Mouse Brain for Persistent and Widespread Neuronal Transduction

Intracerebroventricular and Intravascular Injection of Viral Particles and Fluorescent Microbeads into the Neonatal Brain

Establishing Mouse Models for Zika Virus-induced Neurological Disorders Using Intracerebral Injection Strategies: Embryonic, Neonatal, and Adult

Minimally Invasive Endoscopic Intracerebral Hemorrhage Evacuation

Double Direct Injection of Blood into the Cisterna Magna as a Model of Subarachnoid Hemorrhage

Massive Pontine Hemorrhage by Dual Injection of Autologous Blood

Pre-Chiasmatic, Single Injection of Autologous Blood to Induce Experimental Subarachnoid Hemorrhage in a Rat Model

Read Article