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Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis
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JoVE Journal Cancer Research
Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

DOI:
10.3791/63346-v

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08:15 min

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February 03, 2022

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, , , ,
1School of Biosciences,University of Birmingham, 2Nanomedicine, Drug Delivery & Nanotoxicology Lab, School of Pharmacy,University of Birmingham, 3Mitochondrial Profiling Centre,University of Birmingham

Chapters

  • 00:05Introduction
  • 01:03Probing Mitochondrial Energy Metabolism of Single Spheroids Using XF Technology
  • 02:44Preparation of XF Assay Medium
  • 03:08Preparation of the Sensor Cartridge
  • 04:25Seeding Single Spheroids into the Spheroid Assay Microplate
  • 05:04Preparation and Loading of Compounds into the Sensor Cartridge for XF Assays
  • 05:31Results: Mitochondrial Energy Metabolism in 3D Cancer Cell-Line-Derived Spheroids
  • 07:35Conclusion

Summary

Automatic Translation

Automatic Translation

These protocols will help users probe mitochondrial energy metabolism in 3D cancer cell-line-derived spheroids using Seahorse extracellular flux analysis.

Tags

Keywords: Mitochondrial Energy MetabolismExtracellular Flux AnalysisCell LinesCell NumbersPhenotypesDrug TreatmentsDrug DiscoveryCancer ResearchIn Vitro SpheroidsSpheroid ViabilitySensor CartridgeCalibrantAssay KitPoly-D-LysineXF RPMI Medium

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