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Kvantitativ mikrotubulusfraktioneringsteknik til adskillelse af stabile mikrotubuli, labile mikrotubuli og frit tubulin i musevæv
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Chapters
- 00:05Introduction
- 00:49Isolation and Homogenization of Mouse Tissues to Quantify Microtubule Fractions
- 01:53Fractionation of Mouse Tissues
- 04:04Results: Analysis of the Characterized Murine Microtubule Brain Fractions
- 06:25Conclusion
Mikrotubuli, som er tubulinpolymerer, spiller en afgørende rolle som cytoskeletkomponent i eukaryote celler og er kendt for deres dynamiske ustabilitet. Denne undersøgelse udviklede en metode til fraktionering af mikrotubuli for at adskille dem i stabile mikrotubuli, labile mikrotubuli og frit tubulin for at evaluere stabiliteten af mikrotubuli i forskellige musevæv.
Tags
Keywords Extracted From The Given Text:Quantitative Microtubule FractionationStable MicrotubulesLabile MicrotubulesFree TubulinMicrotubule StabilityAlzheimer’s DiseaseCancerPhosphate Buffer SolutionPBSMicrotubule Stabilizing BufferMSBCentrifugationSupernatantPrecipitateSodium Dodecyl SulfateSDS Sample BufferWestern Blotting
