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Journal / Bioengineering / Affordable Oxygen Microscopy-Assisted…
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Bioengineering

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JoVE Journal Bioengineering
Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids
 

Affordable Oxygen Microscopy-Assisted Biofabrication of Multicellular Spheroids

Article DOI: 10.3791/63403-v • 13:21 min • April 6th, 2022
April 6th, 2022 •
*1, *1, 2, 3, 1
1Tissue Engineering and Biomaterials Group, Department of Human Structure and Repair, Faculty of Medical and Health Sciences, Ghent University, 2Health and Happiness (H&H) Group, National Food Innovation Hub, 3Institute of Analytical Chemistry and Food Chemistry, Graz University of Technology
* These authors contributed equally

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Summary
Automatic Translation

The protocol describes high-throughput spheroid generation for bioprinting using the multi-parametric analysis of their oxygenation and cell death on a standard fluorescence microscope. This approach can be applied to control the spheroids viability and perform standardization, which is important in modeling 3D tissue, tumor microenvironment, and successful (micro)tissue biofabrication.

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Keywords: Oxygen Microscopy Biofabrication Multicellular Spheroids 3D Printing Vascularized Bone Tissue Near-infrared Oxygen Probe PDMS Stamps Agarose Micro-patterning Cell Culture Spheroid Formation
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