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Indsættelse af samfundsforskere til at udføre ikke-destruktiv genetisk prøveudtagning af sjældne sommerfuglepopulationer
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Biology
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JoVE Journal Biology
Deploying Community Scientists to Conduct Nondestructive Genetic Sampling of Rare Butterfly Populations

Indsættelse af samfundsforskere til at udføre ikke-destruktiv genetisk prøveudtagning af sjældne sommerfuglepopulationer

DOI:
10.3791/63416-v

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07:17 min

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October 28, 2022

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, , , , ,
1Department of Entomology and Nematology,University of Florida, 2Florida Museum of Natural History,University of Florida, 3Florida Natural Areas Inventory

Chapters

  • 00:04Introduction
  • 00:59Dissemination of Supplies to Community Scientists
  • 01:33Community Scientist Collection Preparation
  • 02:36Sample Collection in the Field
  • 05:37Results: A Field Method for Non-Destructively Sampling Rare Butterflies for Analyzing Population Genetic Structure
  • 06:36Conclusion

Summary

Automatic Translation

Automatic Translation

Her præsenterer vi en ligetil protokol for ikke-invasiv genetisk prøveudtagning af sommerfuglepopulationer baseret på feltindsamling af resterende ægrestaffald. Det kan bruges til at bekræfte artsidentitet og kvantificere genetisk variation. Denne protokol kan let tilpasses bredere grupper til samfundsvidenskabelig involvering.

Tags

Keywords: Genetic SamplingRare Butterfly PopulationsNondestructive SamplingChorionButterfly OviCommunity ScientistsLow CostEasy To Acquire SuppliesResidual Organic MaterialField CollectionMicrocentrifuge TubesLysis BufferUnique ID LabelsDigital EquipmentField StorageWeatherproof Container

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