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Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms
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Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

Inoculation Strategies to Infect Plant Roots with Soil-Borne Microorganisms

DOI:
10.3791/63446-v

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08:16 min

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March 01, 2022

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,
1Department of Pharmaceutical Biology, Julius-von-Sachs-Institute,Julius-Maximilians-Universität Würzburg

Chapters

  • 00:04Introduction
  • 00:54An in Vitro Inoculation System Based on Petri Dishes
  • 02:55An in Vitro Inoculation System Based on Plastic Cups
  • 04:23A Soil Based Inoculation System in Pots
  • 06:07Results: Infection of Arabidopsis Roots
  • 07:29Conclusion

Summary

Automatic Translation

Automatic Translation

This protocol presents a detailed summary of strategies to inoculate plant roots with soil-borne microbes. Exemplified for the fungi Verticillium longisporum and Verticillium dahliae, three different root infection systems are described. Potential applications and possible downstream analyses are highlighted, and advantages or disadvantages are discussed for each system.

Tags

Keywords: Root InoculationSoil-borne MicroorganismsVerticilliumArabidopsisRoot-microbe InteractionsIn Vitro InoculationRoot InfectionPlant-microbe System

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