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一种高度可扩展的方法,用于对自发 性Caenorhabditis 线虫进行生态调查
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A Highly Scalable Approach to Perform Ecological Surveys of Selfing Caenorhabditis Nematodes
DOI:

09:10 min

March 01, 2022

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Chapters

  • 00:04Introduction
  • 00:34Collection Preparation
  • 01:21Field Collection
  • 02:21Plating Out Field Collections and Isolating Nematodes from Collections
  • 04:06Exporting S-Plates from Fulcrum
  • 04:41Check for Proliferation on S-Plates
  • 05:26Lysis of Isofemale Lines
  • 06:22PCR of SSU and ITS2 Sequences
  • 07:00Identifying Nematodes and Processing the Collection Data
  • 08:04Results: Identifying Nematodes with Sanger Sequencing and Sequence BLAST
  • 08:40Conclusion

Summary

Automatic Translation

该协议可用于对自生 的Caenorhabditis 线虫进行大规模的生态调查。该方法的主要优点是有效地组织和分析与从自然界收集的线虫相关的生态和分子数据。

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