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Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents

JoVE Journal
Biology

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JoVE Journal Biology

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents

Performing In Vivo and Ex Vivo Electrical Impedance Myography in Rodents

DOI:

10.3791/63513-v

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05:44 min

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June 08, 2022

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Marie Mortreux, Janice A. Nagy, Haowen Zhong, Dong-Min Sung, Holly A. Concepcion, Melanie Leitner, Laura Dalle Pazze, Seward B. Rutkove

¹Department of Neurology,Harvard Medical School – Beth Israel Deaconess Medical Center, ²Accelerating NeuroVentures, ³Charley’s Fund

Chapters

00:04Introduction
01:00In Vivo Surface Electrical Impedance Myography
02:19In Vivo Needle Array Electrical Impedance Myography
03:02Ex Vivo Electrical Impedance Myography
04:18Results: Electrical Impedance Myography on Rodent Gastrocnemius Muscle
05:01Conclusion

Summary

Automatic Translation

This article details how to perform in vivo (using surface and needle electrode arrays) and ex vivo (using a dielectric cell) electrical impedance myography on the rodent gastrocnemius muscle. It will demonstrate the technique in both mice and rats and detail the modifications available, (i.e., obese animals, pups).

Tags

Keywords: Electrical Impedance Myography In Vivo Ex Vivo Rodents Muscle Health Biomarker Diagnostic Tool Surface Measurements Needle Array Gastrocnemius Muscle Muscle Pathology Depilatory Cream Saline Solution Contact Impedance Artifacts

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