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Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue
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Bioengineering
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JoVE Journal Bioengineering
Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

Imaging-Guided Bioreactor for Generating Bioengineered Airway Tissue

DOI:
10.3791/63544-v

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11:01 min

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April 06, 2022

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, , , , , ,
1Department of Biomedical Engineering,Stevens Institute of Technology, 2Department of Biomedical Engineering,Columbia University, 3Department of Cell Biology,State University of New York Downstate Medical Center, 4Department of Cardiothoracic Surgery,Stanford University

Chapters

  • 00:05Introduction
  • 01:14Construction of Gradient Index (GRIN) Lens-Based Imaging Device
  • 02:25Imaging-Guided Removal of the Trachea Epithelium
  • 05:51Homogeneous Distribution of Exogenous Cells onto the Denuded Tracheal Lumen
  • 08:23Results: Imaging-Guided Bioreactors for the Generation of Functional In Vitro Airway Tissues
  • 10:18Conclusion

Summary

Automatic Translation

Automatic Translation

The protocol describes an imaging-enabled bioreactor that allows the selective removal of the endogenous epithelium from the rat trachea and homogenous distribution of exogenous cells on the lumen surface, followed by long-term in vitro culture of the cell-tissue construct.

Tags

Keywords: Imaging-guided BioreactorIn Vitro CultivationMicroscopic VisualizationTracheal LumenDe-epithelizationIn Situ ImagingDisease ModelingDrug ScreeningCMOS CameraOptical Cage SystemObjective LensCarboxyfluorescein Succinimidyl Ester (CFSE)PBS WashTrachea CannulaSyringe Pump

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