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Tredimensionelt, serumfrit kultursystem til stamceller fra lacrimalkirtlen
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JoVE Journal Biology
Three-Dimensional, Serum-Free Culture System for Lacrimal Gland Stem Cells

Tredimensionelt, serumfrit kultursystem til stamceller fra lacrimalkirtlen

DOI:
10.3791/63585-v

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07:49 min

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June 02, 2022

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, ,
1MOE Key Laboratory of Gene Function and Regulation, School of Life Sciences,Sun Yat-sen University

Chapters

  • 00:04Introduction
  • 00:47LGSC Primary Culture
  • 03:43LGSC Maintenance and Passage
  • 04:48LGSC Differentiation
  • 05:34Results: Characterization, Differentiation, and Engraftment of LGSCs and Relief of ADDED Symptoms
  • 07:08Conclusion

Summary

Automatic Translation

Automatic Translation

Den tredimensionelle, serumfri dyrkningsmetode til voksne lacrimal kirtel (LG) stamceller er veletableret til induktion af LG organoiddannelse og differentiering i acinar eller duktallignende celler.

Tags

Lacrimal GlandStem CellsThree-dimensionalSerum-freeCulture SystemRegenerationReconstructionExpansion CapacityDifferentiationEuthanized MouseDispaseCollagenase ITrypsin EDTADulbecco’s Modified Eagles MediumHam’s F-12Matrix GelLacrimal Gland Stem Cell Medium

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