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菌根图谱作为探索红费斯图卡和玉米根部定植模式和真菌策略的工具

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Biology

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JoVE Journal Biology

Mycorrhizal Maps as a Tool to Explore Colonization Patterns and Fungal Strategies in the Roots of Festuca rubra and Zea mays

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菌根图谱作为探索红费斯图卡和玉米根部定植模式和真菌策略的工具

DOI:

10.3791/63599-v

•

08:28 min

•

August 26, 2022

•

Vlad Stoian, Roxana Vidican, Larisa Corcoz, Victoria Pop-Moldovan

¹Department of Microbiology, Faculty of Agriculture,University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Chapters

00:05Introduction
00:48Root Processing, Clearing, and Staining for Microscopy
02:10Root Processing for Microscopy
02:56Microscopic Analysis of the Root Samples
03:26Post-Microscopy Image Assemblage and Scoring of Mycorrhizal Colonization
05:05Raw Data Analysis and Result Extraction
06:45Results: Assessment of the Arbuscular Mycorrhizal Colonization Patterns
07:53Conclusion

Summary

Automatic Translation

这里的协议描述了评估丛枝菌根定植模式的方法和两个物种的根策略： Zea mays 和 Festuca rubra。MycoPatt方法的使用允许计算参数，将菌根结构转换为数字数据，以及绘制它们在根中的真实位置。

Tags

Keywords: Mycorrhizal Maps Arbuscular Mycorrhizal Colonization Root Clearing Root Rinsing Root Staining Root Segmentation Microscopic Analysis Festuca Rubra Zea Mays MycoPatt Method

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