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激光显微切割,用于与物种无关的单组织应用
JoVE Journal
Biology
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JoVE Journal Biology
Laser Microdissection for Species-Agnostic Single-Tissue Applications
DOI:

08:57 min

March 31, 2022

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Chapters

  • 00:05Introduction
  • 01:00Removing Mothers and Larvae and Collecting Newly Hatched L1s
  • 02:00Collecting L4 Males and Hermaphrodites and Fixation
  • 03:07Laser Microdissection and Setting up the Microscope
  • 04:27Cutting
  • 06:27Results: Appearance of Caenorhabditis elegans L3 Hermaphrodites and Males, the Focal Plane for the PEN Membrane Slide for Laser Dissection, and Power Analysis Using PowsimR
  • 08:14Conclusion

Summary

Automatic Translation

描述了一种使用激光显微切割来分离单个线虫组织以进行RNA测序的方案。该协议不需要物种特异性遗传工具包,允许在单组织样本水平上比较不同物种之间的基因表达谱。

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