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建立人肺类器官和近端分化以产生成熟的气道类器官

JoVE Journal
Biology

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JoVE Journal Biology

Establishing Human Lung Organoids and Proximal Differentiation to Generate Mature Airway Organoids

Please note that all translations are automatically generated. Click here for the English version.

建立人肺类器官和近端分化以产生成熟的气道类器官

DOI:

10.3791/63684-v

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10:12 min

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March 23, 2022

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Cun Li², Man Chun Chiu², Yifei Yu², Xiaojuan Liu², Ding Xiao², Jingjing Huang², Zhixin Wan², Jie Zhou^2,3

¹Department of Microbiology, Li Ka Shing Faculty of Medicine,The University of Hong Kong, ²Centre for Virology, Vaccinology and Therapeutics,Hong Kong Science and Technology Park, ³State Key Laboratory of Emerging Infectious Diseases,The University of Hong Kong

Chapters

00:04Introuduction
01:16Derivation of Human Lung Organoid
03:25Expansion of Human Lung Organoids
05:31Proximal Differentiation to Generate Mature Airway Organoids
07:42Results: Deriving Human Lung Organoids and Proximal Differentiation to Generate 3D and 2D Airway Organoids
09:38Conclusion

Summary

Automatic Translation

该协议提出了一种从原代肺组织衍生人肺类器官的方法，扩大肺类器官并诱导近端分化以产生3D和2D气道类器官，从而忠实地对人气道上皮进行表型检查。

Tags

Keywords: Lung Organoids Airway Epithelium 3D Culture Cell Isolation Collagenase Digestion Basement Matrix Expansion Medium Proximal Differentiation

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