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In situ Hybridisering kombineret med immunhistokemi i kryosektionerede zebrafiskembryoner
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1School of Life Sciences,Nantong University, 2Medical School,Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration,Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center,Nantong University
Chapters
- 00:04Introduction
- 00:39In Situ Hybridization
- 04:19Embedding and Cryosectioning
- 05:15Immunostaining
- 06:25Results: In situ Hybridization and Immunohistochemistry of Zebrafish Embryonic Sections
- 07:13Conclusion
Denne protokol beskriver, hvordan man opnår billeder ved at kombinere in situ hybridisering og immunhistokemi af zebrafiskens embryonale sektioner. In situ hybridisering blev udført før kryosektion, efterfulgt af antistoffarvning. Det er nyttigt at detektere ekspressionsmønstre af to gener i zebrafisk, hvis der er mangel på antistoffer.
