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使用MALDI-TOF质谱法鉴定酶降解产生的RNA片段

JoVE Journal
Biochemistry

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JoVE Journal Biochemistry

Identification of RNA Fragments Resulting from Enzymatic Degradation using MALDI-TOF Mass Spectrometry

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使用MALDI-TOF质谱法鉴定酶降解产生的RNA片段

DOI:

10.3791/63720-v

•

09:20 min

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April 11, 2022

•

Shawn W. Schowe, Conner J. Langeberg, Erich G. Chapman, Kitty Brown, Marino J. E. Resendiz

¹Department of Chemistry,University of Colorado, Denver, ²Department of Chemistry & Biochemistry,University of Denver, ³Analytical Resources Core Bioanalysis & Omics,Colorado State University

Chapters

00:09Introduction
01:05Concentration Determination of RNA Solution
03:15Hydrolysis of Oligonucleotides of RNA by Xrn-1
05:31Desalting of RNA Solution, MALDI-TOF Plate Spotting
07:41Results: Identification of Enzymatically Degraded RNA Fragments Using MALDI-TOF Mass Spectrometry
08:32Conclusion

Summary

Automatic Translation

MALDI-TOF用于表征从氧化RNA和外切核糖核酸酶Xrn-1之间的反应性中获得的片段。本方案描述了一种可应用于涉及RNA和/或DNA的其他过程的方法。

Tags

Keywords: RNA Fragments Enzymatic Degradation MALDI-TOF Mass Spectrometry Biopolymer Characterization Biochemical Transformations UV-Vis Spectrophotometry Peltier Temperature Control

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