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Standardiserade in vitro-analyser för att visualisera och kvantifiera interaktioner mellan humana neutrofiler och Staphylococcus aureus-biofilmer
JoVE Journal
Immunology and Infection
This content is Free Access.
JoVE Journal Immunology and Infection
Standardized In vitro Assays to Visualize and Quantify Interactions between Human Neutrophils and Staphylococcus aureus Biofilms
DOI:

07:28 min

June 08, 2022

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Chapters

  • 00:04Introduction
  • 00:54Preparation of In Vitro Biofilm
  • 02:34Measurement of ROS Production by Neutrophils
  • 04:14Imaging the Biofilm-Neutrophil Interactions
  • 05:31Results: Assessing Neutrophils and Biofilms Interactions via Standardized In Vitro Assays
  • 06:56Conclusion

Summary

Automatic Translation

Detta protokoll beskriver studien av neutrofil-biofilminteraktioner. Staphylococcus aureus biofilmer etableras in vitro och inkuberas med perifera blod-härledda humana neutrofiler. Det oxidativa sprängresponsen från neutrofiler kvantifieras och neutrofillokaliseringen i biofilmen bestäms genom mikroskopi.

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