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Developmental Biology
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Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis
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Developmental Biology
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JoVE Journal Developmental Biology
Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

Confocal and Super-Resolution Imaging of Polarized Intracellular Trafficking and Secretion of Basement Membrane Proteins During Drosophila Oogenesis

DOI:
10.3791/63778-v

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10:41 min

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May 19, 2022

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,
1Department of Biological Sciences,Northern Illinois University

Chapters

  • 00:05Introduction
  • 00:44Fluorescence Immunostaining
  • 02:28Mounting of Stained Ovaries
  • 04:04Super-Resolution Image Acquisition Using Airyscan Microscopy
  • 07:073D Reconstruction of Optical Z-Section
  • 08:10Results: Characterization of the Intracellular Trafficking and Secretion of Basement Membrane Proteins in Epithelial Cells
  • 10:00Conclusion

Summary

Automatic Translation

Automatic Translation

The basement membrane is essential for tissue and organ morphogenesis during development. To better understand the mechanisms leading to proper placement of this structure, the protocol presented describes methods to visualize and characterize the intracellular trafficking and secretion of basement membrane proteins in epithelial cells using confocal and super-resolution microscopy.

Tags

Keywords: Confocal MicroscopySuper-resolution ImagingPolarized Intracellular TraffickingBasement Membrane ProteinsDrosophila OogenesisFixationImmunostainingFluorescent Secondary AntibodiesAiryscan Microscopy

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