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Chemistry
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从蓝藻中提取非蛋白质氨基酸用于液相色谱-串联质谱分析
JoVE Journal
Chemistry
This content is Free Access.
JoVE Journal
Chemistry
Extraction of Non-Protein Amino Acids from Cyanobacteria for Liquid Chromatography-Tandem Mass Spectrometry Analysis
Please note that all translations are automatically generated.
Click here for the English version.
从蓝藻中提取非蛋白质氨基酸用于液相色谱-串联质谱分析
DOI:
10.3791/63779-v
•
08:14 min
•
December 09, 2022
•
Sercan Pravadali-Cekic
,
Jake P. Violi
,
Simon M. Mitrovic
,
Kenneth J. Rodgers
,
David Bishop
1
Hyphenated Mass Spectrometry Laboratory (HyMaS)
,
University of Technology Sydney
,
2
School of Life Sciences
,
University of Technology Sydney
Chapters
00:05
Introduction
00:44
Raw Sample Preparation
01:56
Cell Lysing and Fractionation of Free Non-Protein Amino Acids (NPAAs)
03:36
Fractionation of the Protein-Bound Non-Protein Amino Acids (NPAAs)
06:11
Sample Filtering
06:35
Results: Extraction of NPAAs from Biological Matrices via TCA Protein Precipitation and Acid Hydrolysis
07:15
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
本协议描述了在使用液相色谱-串联质谱分析之前通过三氯乙酸(TCA)蛋白质沉淀和酸水解
从
生物基质中提取非蛋白质氨基酸。
Tags
Non-protein Amino Acids
Cyanobacteria
Liquid Chromatography-tandem Mass Spectrometry
BMA
AEG
2
4-DAB
Extraction
Freeze Drying
Sonication
Centrifugation
Trichloroacetic Acid
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