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用于检测人类精子功能缺陷的生物标志物的流式细胞术分析
JoVE Journal
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JoVE Journal
Medicine
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Please note that all translations are automatically generated.
Click here for the English version.
用于检测人类精子功能缺陷的生物标志物的流式细胞术分析
DOI:
10.3791/63790-v
•
08:48 min
•
April 21, 2022
•
Xi Ling
,
Peng Zou
,
Lin Ao
,
Niya Zhou
,
Xiaogang Wang
,
Lei Sun
,
Huan Yang
,
Jinyi Liu
,
Jia Cao
,
Qing Chen
1
Institute of Toxicology, College of Preventive Medicine
,
Army Medical University (Third Military Medical University)
,
2
Department of Chemical Defense Medicine, College of Military Preventive Medicine
,
Army Medical University (Third Military Medical University)
Chapters
00:04
Introduction
01:01
Cell Preparation and Sperm Cells Counting Using Computer‐Aided Sperm Analysis (CASA)
01:37
Staining Sperm Cells
04:52
Flow Cytometer Setup
07:10
Results: Flow Cytometric Analysis of Sperm Function Markers for Detecting Human Sperm Functional Defect in a Single Cytometry
08:22
Conclusion
Summary
Automatic Translation
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Automatic Translation
本方案提供了一种实用的解决方案,允许使用单个细胞仪测量人类精子中的细胞凋亡、线粒体膜电位和DNA损伤。
Tags
Flow Cytometry
Sperm Functional Biomarkers
Sperm Apoptosis
Mitochondrial Membrane Potential
Sperm Function Analysis
Infertility Diagnosis
Semen Analysis
JC-1 Probe
CCCP
Annexin-V FITC PI Staining
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