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Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
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JoVE Journal Cancer Research
Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid

Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid

DOI:
10.3791/63817-v

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07:24 min

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May 25, 2022

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, , , , , ,
1Department of Pathobiology,University of Guelph

Chapters

  • 00:04Introduction
  • 00:55Purification of NDV from Allantoic Fluid
  • 05:08Quality Control Assays
  • 05:55Results: Production of High-Titer Recombinant NDV
  • 06:53Conclusion

Summary

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Here we provide a detailed procedure for production, purification, and quantification of high-titer recombinant Newcastle disease virus. This protocol consistently yields > 6 × 109 plaque-forming units/mL, providing virus quantities appropriate for in vivo animal studies. Additional quality control assays to ensure safety in vivo are described.

Tags

Keywords: Newcastle Disease VirusRecombinant VirusVirus PurificationTangential Flow FiltrationAllantoic FluidDepth FiltrationBuffer ExchangePressure ControlVirus Concentration

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